FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578901
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA211190.5900973511142107No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC180290.5037579972175816No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC135500.37860784637518613No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT94800.26488578477024094No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC55780.15585790162957847No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC54480.1522255016274549No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47040.131436996999917No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC44350.12392072314936903No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42920.11992508314703312No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41920.11713092929924578No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG41770.11671180622207766No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC41180.11506325545188315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG9550.056.9711045
TACGGCC9650.056.023897
GTCTACG9750.055.8009034
CCGAACT12900.053.318967
GCGCGGT77250.052.8227239
CGGTTCA13150.052.1108635
GCCGGTT13100.052.045443
CTACGGC10400.051.3214956
GCCATAC10500.051.16066711
CGCGGTG81200.050.42277510
ACTCTTC47500.050.3114475
TCTGGTC47800.050.13976710
GGGGACT45700.049.717231
TGGCGCG82900.049.3887815
TTCTGGT50200.047.8798339
GGACTCT53200.047.5087053
CCGGTTC14500.047.495314
TCCTTAG14350.047.27322816
TTAGGCA14450.047.21410419
CGAACTT15100.046.86657368
Produced by FastQC (version 0.11.5)