FastQCFastQC Report
Wed 8 May 2024
EGAF00005317859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666867
Total Bases274.6 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT226270.6170662857420245No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG149310.4071868437006305No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTG64900.17699032989197588No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA63100.17208150718310755No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC59090.1611457410372397No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCC52160.1422467736080965No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT50130.13671071244198385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46480.1267567108378897No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45130.12307509380623842No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT43400.11835716975827047No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT42410.11565731726839287No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA41110.11211205642309907No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC37710.10283983575079216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)