FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666867
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA139470.3803519462254835No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC118910.3242822823952982No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC86870.2369052381774414No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA60600.16526369786523482No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT60100.16390013600166026No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC59060.1610639273254252No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46480.1267567108378897No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG45650.12449319814435593No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT43370.118275356046456No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42880.11693906542015295No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA40580.11066668084771004No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC37380.10193988492083296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT51950.051.752929
CGGTTCA9600.049.5495385
ACTCTTC48650.048.7458155
TGGCGCG55850.048.5066715
CCGAACT9950.048.40995467
GGGGACT44800.048.3427621
CGCGGTG56050.048.02874810
TCTACGG5850.046.548215
TCTGGTC50200.046.4161210
GGACTCT53100.046.219283
GGGACTC50850.046.0951922
TACGGCC5950.045.7652637
GCCGGTT10600.045.200853
TTCTGGT51800.045.1820379
TGGTCCG15050.043.9734512
TGTAGTC62850.043.59460426
GTAGTCC63100.043.4361527
CCGGTTC11150.043.2797664
TCTTCTG54900.043.195847
TAGTCCC64050.042.91803728
Produced by FastQC (version 0.11.5)