FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673610
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA134220.36536268139513994No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113820.30983147367303554No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC85040.2314889168964588No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT58170.15834560554876537No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA58060.1580461725659501No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53990.14696715220178516No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47130.12829342254621476No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43960.11966430840508382No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41330.1125051379977733No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC40550.11038188593781048No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA40310.10972857761166808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT51800.052.4357539
TACGGCC7500.051.4739887
TCTACGG7500.051.015795
GGGGACT45450.049.5796131
ACTCTTC50300.048.9297645
TGGCGCG56100.048.658415
CCGAACT10650.048.3105967
CGGGACT12150.048.083381
CGCGGTG56750.047.922810
CGGTTCA10650.047.9021455
TCTGGTC50600.047.54840510
GGACTCT52400.047.4925083
GCCGGTT10700.047.031443
GTCTACG8100.046.808734
GGGACTC53150.045.9937022
CTACGGC8450.045.6882136
TTCTGGT52850.045.3936739
TGGTCCG14400.044.9989812
CCGGTTC11750.044.0020184
TCTTCTG56950.042.9729967
Produced by FastQC (version 0.11.5)