FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625398
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG217890.60100987532955No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC101500.27996926130593114No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG94650.2610747840650875No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC87720.24195964139661358No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG64790.17871141320208153No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG60860.16787122406974353No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC60050.16563698661498683No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG55840.15402446848594278No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA48760.13449557814066207No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG45190.12464838343266035No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT42390.11692509346560018No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT39890.11002929885215362No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39390.1086501399294643No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC38440.1060297379763546No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT37870.10445749680448876No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG37440.10327142013097595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA61150.039.50507432
GCGGCAC63450.038.19235233
CGGCACC64950.037.16253334
CCGTCGC63150.036.17649545
GTTATCG2300.036.057626
TCGCCGG64050.035.72879848
GCACCCC67950.035.59404436
CCCGTCG64750.035.48042344
AGGACCG7400.034.7400970
GTGCCGT5700.034.6768071
TAGCGTC6450.034.28782711
GGCACCC70550.034.2685135
CACCCCC72100.033.79904637
GTTCCGT3800.033.764261
CCCCCGA72350.033.63502519
CCCCCGT71100.031.9956542
TAGTCCG4600.031.5504196
GTAGCGT7050.030.8795610
ACCCGCG79600.030.64669425
TATCGGA2500.030.4085968