FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613843
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC157010.4344682378288155No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC119950.331918127046471No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT81170.22460853999468158No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA75590.2091679134926448No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56090.1552087348564949No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC43140.11937430596735939No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG39880.11035343815434151No Hit
GGGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGC38180.10564930463221563No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT36800.10183065506719577No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36440.10083448561545147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC8100.055.4055637
GCGCGGT71250.053.7330789
CCGAACT12550.051.5728167
GTCTACG8750.051.2904324
TGGCGCG75600.051.09510415
CGCGGTG75500.050.84554310
CGGTTCA12650.050.4873125
GCCGGTT13100.049.016553
TCTACGG9200.048.0311745
CGAACTT14650.046.4409268
GCCGAAC14400.044.9829766
CTACGGC10050.044.6539966
CCGGTTC14350.044.5062374
GAACGCG10000.043.89756424
CGGCAAT3450.043.3104361
CTTAGGC14850.043.2485718
GGCGCGT90600.043.0172616
TGAACGC10300.042.9450523
CGTGCCT90450.042.7658420
TGTAGTC91050.042.345426