FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3614110
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG216680.5995390289725548No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC101820.281729111731519No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG93360.25832085907733854No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC88640.24526093561070358No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG65660.18167681669899366No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG63730.17633663612895015No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC59150.1636640832736137No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG58910.16300001936853056No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA48210.13339383693357423No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG43740.1210256467014009No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT41860.11582381277824969No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40970.11336124246356641No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39180.10840843250482138No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC38640.10691428871838432No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG37740.1044240490743226No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT37000.10237651870031626No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG36700.10154643881896235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA63600.037.04678732
GCGGCAC66100.035.81532733
CGGCACC67250.035.2165834
CCGTCGC65700.034.19830345
GCACCCC70300.033.71682436
TCGCCGG66450.033.70260248
GTTCCGT3450.033.312561
CCCGTCG68050.033.0495844
AGGACCG7850.032.33013570
CACCCCC74100.032.1407837
CCCCCGA74800.032.0071419
GGCACCC73750.031.97925435
CCCCCGT73000.030.68403242
ACCCGCG80400.029.377425
CCCCGAC82150.029.1050220
GTGCCGT5200.028.7990591
CGCCCTA12650.027.56021161
TAGTCCG4150.027.4562036
TTATCGG2800.027.1293437
CTTACCC13250.027.04105470