FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260202
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG189720.5819271321224881No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100350.30780301343291No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG68200.20918949193945652No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG52730.16173844442767657No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51050.1565853894942706No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC46970.14407082751314182No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC40010.12272245707474567No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT39730.12186361458584467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA56950.036.27884732
GCGGCAC58650.035.18301833
CGGCACC60450.034.2117734
TATCGGA1650.033.280278
TCGCCGG58300.033.0029948
CCGTCGC59500.032.44053345
CCCGTCG59950.032.2270244
AGGACCG7350.032.10251270
TTATCGG1950.031.6802567
GCACCCC66000.031.42296436
AGTCCGC67600.030.8679437
CACCCCC67900.030.51109537
CCCCCGA67050.030.49418819
CAGTCCG65800.030.4083926
GTCCGCC70100.029.9139678
CGTACGT2300.029.88140719
ACCCGCG69400.029.53300925
CGATCTA2500.029.36514547
TCCGCCC72100.029.3208269
CCCCCGT65850.029.29743242