FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260202
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99460.3050731212360461No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA79720.24452472576852602No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC73650.22590624752699373No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC58330.17891529420569646No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT33940.1041039788332134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT35000.050.670249
TGGCGCG37000.048.0232815
CGCGGTG39100.045.4447210
CCGAACT7400.042.64895667
CGGCATA2350.041.0210341
CTACTCG43450.040.72887437
TACTCGG43250.040.61082538
GCCGGTT7900.039.515263
GTAGTCC45900.038.97332827
CGGTAAG3150.038.253741
ACTCGGG45950.038.25372739
TAGTCCC47000.038.23444428
TGTAGTC47200.038.1048526
CGTGCCT47100.038.08220720
CGGTTCA8150.037.8798875
GTACGCA4750.037.48624465
CCTGTAG49700.036.2886424
GGGGTAG15000.036.2645451
AGGATCG47750.036.22665459
GATCGCT48050.036.22413361