FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258877
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG201000.6167768835706288No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102020.31305262518346044No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG72360.22203967808542635No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG55080.16901527734860813No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG54430.16702072523755881No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC45430.13940384985379933No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT42040.1290014934592499No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC38910.11939695790912024No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC32740.10046405556269843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57300.036.48270832
GCGGCAC59400.035.2109433
CGGCACC59950.034.79290434
TCGCCGG58600.034.20537648
CCGTCGC59550.033.74756645
CCCGTCG60750.033.1755844
CGATCTA2500.032.1536647
GCACCCC66150.031.82887536
ACCCGCG68100.031.08554825
CCCCCGA68250.030.9777519
AGTCCGC68500.030.9727787
GTTATCG2250.030.516156
CCCCGAC69250.030.4939320
CACCCCC69400.030.45817837
CAGTCCG65900.030.3714436
CCCCCGT66250.030.32183542
GGCACCC69650.030.26135835
CGTACGT2650.029.8372919
TCCGCCC72750.029.399329
GTCCGCC72300.029.392378