FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355303
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102130.30438383657154067No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA87130.2596784850727341No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76240.22722239988460058No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61380.18293429833311628No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT35390.1054748259695175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT37150.051.1735889
TGGCGCG39550.047.79936215
CGCGGTG41300.046.11453610
CCGAACT7000.043.53632767
TACTCGG45300.042.98432538
GCCGGTT6950.042.9488373
CTACTCG45400.042.93557737
CGGCATA2550.041.855641
CGGTTCA7450.040.5268975
TAGTCCC47900.040.3328528
ACTCGGG48400.040.2512939
CGGGTAG4800.040.167911
GTAGTCC48650.039.6931727
TGTAGTC49600.039.12384826
CGTGCCT49400.038.4735720
GGGGACT32300.038.16041
CCTGTAG51350.037.5536124
AGGATCG49450.037.45803559
GTACGCA4850.037.4203665
GATCGCT50800.036.68992661