FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4153396
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174110.41919913246894824No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC107980.25998002598355663No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC88490.2130545702841723No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA72120.17364103976601317No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT50950.12267070127673836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT55750.051.3840879
CCGAACT9850.050.15095567
TCGCGTA800.049.11999560
TGGCGCG59950.047.6721615
CGGTTCA10100.046.259265
CGCGGTG64650.044.46858210
GCCGGTT10600.044.0820163
CTACTCG67500.042.57237237
TACTCGG67700.042.3434738
GTAGTCC69250.041.499227
TAGTCCC69300.041.2541728
TACGGCC5600.040.1927
TGTAGTC72100.040.06666626
ACTCGGG74150.039.54060739
CGTGCCT73000.039.42691420
CCGGTTC12050.039.056344
CCTGTAG74450.038.73977324
GCTACTC76350.037.6812336
CGGTAAG3000.037.6614231
AGTCCCA76650.037.3786429