FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4179001
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG266530.6377840062732696No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176660.42273261001851875No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG110110.26348402405263843No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG72580.17367787181673322No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA58330.13957881321397148No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC56460.13510405955873186No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG56030.13407510550966606No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC55900.13376402637855317No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG48230.1154103576428912No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC48190.11531464098716415No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC47880.11457283690527952No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC44330.10607798370950378No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG44300.10600619621770849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA78100.036.39752632
GCGGCAC80400.035.34173233
TCGCCGG79000.034.18543648
CCGTCGC79250.034.12527545
CCCGTCG80050.034.0448744
CGGCACC83600.033.89678234
GCACCCC86900.032.7018736
CCCCCGA89250.032.0893719
CACCCCC91650.031.19649137
ACCCGCG92200.030.9497725
GTGCCGT6650.030.8194641
AGGACCG10050.029.72508270
GGCACCC96800.029.40414235
AGTCCGC103200.028.3662417
TAGTCCG5000.027.977116
CAGTCCG100600.027.8102476
GTCCGCC106450.027.6284058
CCCCGAC104350.027.36276420
TCCGCCC109400.027.3512069
CGTCGCC99950.027.22667546