FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4179001
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174790.41825785636327917No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC112250.2686048651340356No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC93830.22452734517172884No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA75400.18042589604549028No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51910.12421628996977988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT58500.050.8481069
CCGAACT10100.049.2524867
TGGCGCG63750.046.3919515
CGGTTCA10600.044.7324945
TACGGCC5200.044.608727
GCCGGTT10650.044.2021753
CGCGGTG67850.044.1405710
CTACTCG71800.041.93924337
TACTCGG72700.041.6092638
GTAGTCC73500.040.9290527
TAGTCCC74750.040.28018628
CTTAGGC11900.040.17972618
GTCTACG5900.039.8943374
TGTAGTC76300.039.5300526
ACTCGGG78400.038.9766639
CGGGTAG5650.038.7646681
CGTGCCT76200.038.5406320
CCTGTAG78750.038.205424
GGTACGA3000.037.5238043
GCTACTC80750.037.3297636