FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286848
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN179830.4194923636200771No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113370.26446004150368757No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC92510.21579958048430922No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA80510.18780698545877997No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT52570.12263089337433937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC5650.051.3665247
GCGCGGT57900.051.126999
CCGAACT10350.047.6734567
TGGCGCG63050.047.26306515
TCTACGG6250.044.78495
CGGTTCA10800.044.5649225
CGCGGTG67750.044.04654310
TACTCGG73500.041.67775738
CTACTCG73350.041.6292637
GTCTACG6900.041.555454
GCCGGTT11850.041.1917653
GTAGTCC74200.041.0187327
TAGTCCC74750.040.6537528
TGTAGTC75800.040.2188926
CCTGTAG77950.038.9737724
CGAACTT13900.038.8156668
ACTCGGG80650.038.5865739
CGTGCCT78150.038.25483720
CTACGGC7600.038.180696
CGGGTAG5300.037.477091
Produced by FastQC (version 0.11.5)