FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236946
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174450.4117352451506345No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113160.267079165040102No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC90270.2130544028647049No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA79150.18680908371265528No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT53860.12711986416631224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT56350.050.191589
CCGAACT10000.048.65651367
TGGCGCG60250.046.65954215
GCCGGTT10500.044.839433
CGCGGTG64750.043.83831410
TACGGCC5450.043.8204657
CGGTTCA11150.042.225975
TCTACGG5650.041.0616045
CTACTCG71200.040.7425237
TACTCGG71650.040.3706938
TAGTCCC71350.040.32286528
GTCGTAC1950.040.2405133
GTAGTCC71750.039.82905227
GTCTACG6100.039.1506084
TGTAGTC74350.038.8246826
CCTGTAG75200.038.4303924
CGGGTAG6250.038.36651
ACTCGGG77250.038.3433839
CGTGCCT75250.038.216820
CGGCATT6650.038.119241