EGAF00005317888 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005317888
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501000
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	6268	1.2510978043912175	No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG	3684	0.7353293413173653	No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG	1871	0.37345309381237524	No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG	1375	0.27445109780439125	No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC	1345	0.2684630738522954	No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT	1195	0.23852295409181634	No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT	1061	0.21177644710578844	No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC	1047	0.2089820359281437	No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA	1000	0.19960079840319359	No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG	948	0.18922155688622755	No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG	766	0.15289421157684632	No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT	748	0.14930139720558883	No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT	730	0.14570858283433133	No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG	720	0.1437125748502994	No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA	676	0.13493013972055887	No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG	660	0.1317365269461078	No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG	602	0.12015968063872255	No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA	600	0.11976047904191617	No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG	561	0.11197604790419162	No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC	557	0.11117764471057885	No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC	555	0.11077844311377245	No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT	547	0.10918163672654692	No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG	512	0.10219560878243514	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGG	30	1.034675E-5	56.286465	7
GCCGCTA	20	0.0079687135	50.714188	1
TCGTATG	70	7.2759576E-12	49.935978	40
TACTCGA	70	7.2759576E-12	49.29663	31
TTACTCG	70	9.094947E-12	49.240704	30
GTACGTA	30	6.783113E-4	45.32689	20
TATCGGA	30	7.0069847E-4	45.02917	8
GTGTTCG	70	5.456968E-10	43.420986	14
TAGTCCG	50	4.444346E-6	40.52625	6
CCAGCGT	300	0.0	39.124287	70
TACGTAC	55	5.765656E-6	39.0185	56
CTACGTA	55	5.799644E-6	38.985126	55
CGTATGC	90	1.03682396E-10	38.900124	41
GTGCCGT	80	1.9954314E-9	38.03564	1
CGCGGCA	1185	0.0	37.0479	32
TATGCCG	95	1.8007995E-10	36.965046	43
CGCGGGA	65	6.376631E-7	36.410187	1
TCGCAGA	85	3.5488483E-9	35.85992	18
TGTTCGC	85	3.6488927E-9	35.75846	15
CCGATCG	200	0.0	35.72218	70

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content