Basic Statistics
Measure | Value |
---|---|
Filename | EGAF00005317889 |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 501000 |
Sequences flagged as poor quality | 0 |
Sequence length | 35-76 |
%GC | 59 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 6155 | 1.2285429141716566 | No Hit |
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA | 2460 | 0.4910179640718563 | No Hit |
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC | 1781 | 0.35548902195608784 | No Hit |
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC | 1287 | 0.2568862275449102 | No Hit |
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT | 674 | 0.1345309381237525 | No Hit |
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA | 577 | 0.1151696606786427 | No Hit |
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG | 559 | 0.11157684630738524 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGGCGTA | 30 | 1.0168442E-5 | 56.45183 | 1 |
GCCGGTT | 105 | 0.0 | 51.451473 | 3 |
GGCGTAT | 20 | 0.007911536 | 50.806644 | 1 |
CCGAACT | 115 | 0.0 | 50.701 | 67 |
GCGCGGT | 850 | 0.0 | 48.06551 | 9 |
TCTACGG | 50 | 8.215284E-8 | 47.271038 | 5 |
TGGCGCG | 880 | 0.0 | 46.810608 | 15 |
GGGGACT | 635 | 0.0 | 45.87267 | 1 |
GCGTCAA | 60 | 6.7739165E-9 | 45.02003 | 3 |
TACTCGG | 930 | 0.0 | 43.87016 | 38 |
CGGGTAG | 70 | 5.293259E-10 | 43.548557 | 1 |
CGCGGTG | 965 | 0.0 | 43.387184 | 10 |
ACTCTTC | 670 | 0.0 | 43.340183 | 5 |
CTACTCG | 945 | 0.0 | 43.110844 | 37 |
CGGTACC | 55 | 1.6957347E-7 | 43.108673 | 1 |
TAGTCCC | 950 | 0.0 | 43.03861 | 28 |
TCTGGTC | 660 | 0.0 | 42.97367 | 10 |
ACTCGGG | 980 | 0.0 | 42.387768 | 39 |
GGGACTC | 695 | 0.0 | 42.271282 | 2 |
TGTAGTC | 975 | 0.0 | 41.823273 | 26 |