FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501000
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61551.2285429141716566No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA24600.4910179640718563No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC17810.35548902195608784No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC12870.2568862275449102No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT6740.1345309381237525No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA5770.1151696606786427No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG5590.11157684630738524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA301.0168442E-556.451831
GCCGGTT1050.051.4514733
GGCGTAT200.00791153650.8066441
CCGAACT1150.050.70167
GCGCGGT8500.048.065519
TCTACGG508.215284E-847.2710385
TGGCGCG8800.046.81060815
GGGGACT6350.045.872671
GCGTCAA606.7739165E-945.020033
TACTCGG9300.043.8701638
CGGGTAG705.293259E-1043.5485571
CGCGGTG9650.043.38718410
ACTCTTC6700.043.3401835
CTACTCG9450.043.11084437
CGGTACC551.6957347E-743.1086731
TAGTCCC9500.043.0386128
TCTGGTC6600.042.9736710
ACTCGGG9800.042.38776839
GGGACTC6950.042.2712822
TGTAGTC9750.041.82327326