FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154423
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG86840.2752959891555445No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG35490.11250869017883779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA28600.036.85270732
GCGGCAC31600.033.801533
CCCGTCG30150.032.65278244
TCGCCGG30400.032.55216648
CGGCACC33200.032.07570634
CCGTCGC31600.031.16342245
ACCCGCG36150.029.98383725
CCCCCGA36200.029.82592219
GCACCCC38550.027.90613736
CCCCGAC38400.027.85076720
CACCCCC40750.026.2375837
AGTCCGC41450.026.124247
CAGTCCG40600.025.733856
CCGCGCG42000.025.32044627
TCCGCCC44400.025.1676929
CCCGACC42750.025.10137621
GGCACCC42700.025.02742635
GTCCGCC44500.024.5669338
TAGCGCG2101.5072146E-824.45469770
AGGACCG5700.024.32598970
Produced by FastQC (version 0.11.5)