FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139568
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG86190.2745282153468248No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG35000.11148030557070272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA28000.035.42134532
TCGCCGG27700.034.71161348
CCCGTCG28800.033.09292244
GCGGCAC30600.032.644633
CCGTCGC29700.032.09987645
CGGCACC31700.031.5183734
GTACGGC3600.029.79234714
CCCCCGA34950.028.81711619
AACGAGC3750.028.58817970
ACCCGCG35300.028.54868725
GGCGATC6000.027.22683770
CCCCGAC37150.027.20504820
GCACCCC38600.026.16600836
CGCCCTA7800.025.63495461
AGTCCGC39650.025.3906927
CACCCCC39550.025.36846737
TACGGCC4200.024.71254315
GTGCCGT5800.024.672741
AGGAGGG59100.024.44541470
GGCACCC41450.024.44398935
Produced by FastQC (version 0.11.5)