FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139568
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA2400.039.1504061
CGGCAAT4050.037.80781
GCGCGGT15750.036.8891229
CGGGTAG5150.035.138231
CGCGAAT501.8319205E-434.596232
TGGCGCG16700.034.584115
GGGGGGA66200.034.4849551
CGGCTAT3350.034.2809561
CGGCAAC6000.033.640351
GGGGTAT13750.033.1552541
GGGGTAG16000.033.0603451
CGGTACG851.12091584E-732.7532841
CGGTAAG2800.032.314621
CGGTATT3800.032.0529671
TTACGCG553.1623102E-431.5239834
CGCGATA450.004431905730.9336551
CTACTCG18900.030.47841337
CGGATAT2650.030.2040861
TACTCGG18900.030.1193938
GGGGGAT34800.030.1003151
Produced by FastQC (version 0.11.5)