FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134668
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG85060.27135250048808995No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG36770.11730109855333962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA25700.034.4088132
GCGGCAC29100.030.3919433
TCGCCGG26200.030.3249448
CCGTCGC27000.029.2592545
CCCGTCG27250.029.23732244
CGGCACC30200.029.1738434
CCCCCGA32400.027.01967419
ACCCGCG33100.026.88917225
GCACCCC33200.026.87216236
CGCCCTA7450.026.8484561
CCCCGAC33750.025.94095820
GACGCGT1101.083512E-625.27262340
GGCACCC37900.024.07734135
CACCCCC37700.023.7631637
AGGACCG5650.023.44877870
GTGCCGT5250.023.238861
CCCGACC38400.023.1627321
AGTCCGC38300.023.0292427
CAGTCCG37250.022.8426786
GTTCCGT2450.022.7645971
Produced by FastQC (version 0.11.5)