FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493966
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG158730.45429749459496743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76630.2193209664890843No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59700.17086600155811477No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43000.12306931435509103No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC37660.10778582275843554No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37470.10724202811361072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA48350.036.5782332
GCGGCAC50300.035.2438733
CCCGTCG47900.034.86165244
TCGCCGG48550.034.03070448
CCGTCGC49250.033.6419445
CGGCACC52950.033.55521834
ACCCGCG55400.032.3332325
CCCCCGA58050.030.91541919
GCACCCC60400.029.84569236
CCCCGAC61150.029.41473820
CACCCCC62650.028.73041537
AGGAGGG82750.028.39140170
CGTACGT2200.028.23734319
GGCACCC64350.027.84450135
AGTCCGC68150.026.7177147
CCCGACC68000.026.40920621
CAGTCCG67600.026.2209036
CCGCGCG69600.025.85595927
CGTACCG1900.025.75289249
TCCGCCC73500.025.3810759
Produced by FastQC (version 0.11.5)