FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493245
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG167770.4802697778140383No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75360.21573064586079704No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG64000.183210739584541No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44770.12816163767499847No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA40350.11550864597244109No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC38260.10952567025788343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52550.036.15388532
GCGGCAC54800.034.49209633
TCGCCGG53050.033.50388348
CGGCACC56650.033.38350334
CCCGTCG53650.033.314144
CCGTCGC55500.032.03571345
ACCCGCG59300.032.02870625
CCCCCGA62350.030.73960519
AGGAGGG82750.030.48062170
GCACCCC63050.030.28882636
CACCCCC63600.029.65500337
CCCCGAC65700.029.17849520
TAGTCCG3950.028.8280876
CGTACCG2500.027.95676649
GGCACCC69000.027.66697735
AGTCCGC71250.026.878637
TCGTACG4250.026.79316312
TCCGCCC75850.026.2493929
CAGTCCG69550.026.0472076
CCGCGCG73650.025.89259127
Produced by FastQC (version 0.11.5)