FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493245
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74390.21295385808896886No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC49650.14213145656831971No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47900.1371217879078049No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC39390.11276048487867299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA3300.047.185561
GCGCGGT26750.046.1659749
TGGCGCG27250.044.9371915
CTACTCG31850.038.5950737
TACTCGG32150.038.3545738
GGCGATA3000.038.0630151
CCGAACT6350.037.45034867
GTAGTCC35200.036.06950427
CGCGGTG34300.036.00045810
TAGTCCC35550.035.6324528
CGGGTAT4000.035.467811
AGGATCG33800.035.03884559
ACTCGGG35800.034.7492539
CGGGTAG5350.034.279351
GATCGCT34750.034.13032561
TGTAGTC37800.033.58080326
GTGGCGC37450.033.4328914
CGTGCCT37400.033.2361920
CGGCATT7200.033.160961
GCCGGTT7050.032.2941473
Produced by FastQC (version 0.11.5)