FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573414
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG169160.47338483590202535No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74210.20767255067562843No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63800.1785407456286901No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44710.1251184441545256No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA41430.11593954688709453No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC40570.11353288479868272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51750.034.1803232
GCGGCAC53800.032.82454333
TCGCCGG51400.032.2012148
CCCGTCG52500.031.65197844
CCGTCGC53050.031.276345
CGGCACC57350.030.92370234
ACCCGCG58200.030.44243225
CCCCCGA61250.029.15695819
AGGAGGG84550.028.53077570
GCACCCC63250.028.33104736
CACCCCC63400.028.32958237
CCCCGAC65550.027.19770420
GGCACCC68600.026.11418735
CGCCCTA10650.024.77461
AGTCCGC71700.024.7452057
CAGTCCG70250.024.3223726
GTGCCGT8400.024.2795931
CCGCGCG73150.024.18638627
CCCGACC74350.023.98534221
TCCGCCC77250.023.9504329
Produced by FastQC (version 0.11.5)