FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573414
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73270.20504201304410852No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC48930.1369278790534766No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48650.13614431465259832No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC38210.10692855627699449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT29250.042.829629
TGGCGCG32600.038.633115
CGGGTAG4600.037.6275021
CGGTATA2050.037.1502761
CTACTCG33850.037.14125437
CGGCATA3200.036.7808841
CGGCAAT3700.036.488511
TACTCGG34650.036.2955238
CGGTATT2850.035.2246251
AGGATCG35100.034.92359559
CGGTATC2400.034.6173021
GATCGCT35700.034.28425261
CGCGGTG37300.034.14137610
CGGTAAG3300.033.5682951
ACTCGGG38900.033.32612239
TAGTCCC39100.032.8475228
GTAGTCC39100.032.65804327
GGGGTAT13000.032.4870071
CGGGGTA3200.032.453721
CGTGCCT39450.032.13186320
Produced by FastQC (version 0.11.5)