FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332565
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG156920.470868535197363No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG49400.1482341679757184No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39170.11753709229977509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA45800.038.65164632
GCGGCAC48550.036.5404133
TCGCCGG46100.034.7562448
CGGCACC51600.034.18540634
CCCGTCG48250.033.7412144
CCGTCGC48000.033.41720245
ACCCGCG53800.033.11640525
CCCCCGA55050.032.5854519
AGGAGGG72850.032.30258670
CCCCGAC56150.031.95140820
AGTCCGC56450.031.8252727
CAGTCCG54650.031.0999776
GCACCCC58600.030.58646236
CACCCCC58650.030.44606237
TCCGCCC59850.030.364349
CCGCGCG58800.030.2573527
GTCCGCC60600.029.7029428
CCCGACC63200.028.33666221
GCGCGGG57050.028.11642662
CGTCGCC57550.027.88027846
Produced by FastQC (version 0.11.5)