FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332565
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC33590.10079323284016967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT18200.042.220339
TGGCGCG19650.039.1054215
GGGGGGA63500.034.7177051
CTACTCG22450.034.34308637
CGGCACT7350.034.116631
TACTCGG22900.033.82803338
AGGATCG22750.033.20379359
CGGCATA2950.033.056511
CGGCATT6450.032.937541
GGGCATA11100.032.6310731
GGGGTAT14900.032.4899861
GGGGTAC10950.032.4419561
GGGGTAG19650.032.4346731
CGGCAAT3800.032.0778661
CGGCAAA7850.031.4999371
GATCGCT24050.031.29205561
GGGGTGT30950.031.2827641
CGCGGTG24800.030.98427410
CGGGAAT7650.030.9576871
CGGGTAG6050.030.509951
Produced by FastQC (version 0.11.5)