FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337197
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG157910.4731815352824541No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG49200.14742911491290445No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39940.11968127743132934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA47400.039.02169832
GCGGCAC49900.037.07784733
TCGCCGG48450.035.3099848
CCCGTCG49700.034.45146644
CGGCACC53750.034.17090234
CCGTCGC50600.033.70520445
ACCCGCG55650.033.32196825
CCCCCGA56600.033.04506719
AGGAGGG77300.033.0072970
AGTCCGC57400.032.8170137
CCCCGAC58250.032.1119120
CAGTCCG55150.031.7699936
GCACCCC58400.031.58087336
TCCGCCC60600.031.2555269
CCGCGCG59850.031.11342227
CGTACGT1450.031.05307819
CACCCCC60950.030.55419337
CGCCCTA7350.030.47979261
GTCCGCC61700.030.473818
TAGTCCG4500.030.0098746
Produced by FastQC (version 0.11.5)