FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366145
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA163510.48574853430259246No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG154500.4589820105788669No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC153250.45526856389133563No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC129850.38575284190074993No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120320.3574415243550115No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT70460.20931956288276354No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG48620.1444382223582169No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC39190.11642398054748086No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34610.10281791188436624No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA34190.10157019379735573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG34600.062.617064
TCTACGG34700.062.3379755
CTACGGC35950.060.075246
TACGGCC35700.060.018367
ACGGCCA38800.055.3112958
CGGCCAT39300.054.5204969
GGGTCTA29300.054.433212
GCGTCTA8050.053.994992
GCCATAC40000.053.64475611
GCGCGGT74650.052.9119769
CATACCA40950.052.3213613
CCATACC41400.052.00066412
CCGAACT11550.050.7323567
GGCCATA42600.050.6184610
CGCGGTG79550.049.65278610
TGGCGCG79950.049.48556515
TACCACC44650.048.5200715
CGTCTAC9950.048.4999923
ATACCAC44850.048.38001314
GCCGGTT11700.047.9737433