FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384058
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG153220.4527700175351605No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG146760.4336805102040213No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC131290.38796616370050396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122490.36196188126799245No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC103630.30622997596376894No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG68130.20132633660534188No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA63510.18767408832827334No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT58000.1713918614870076No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT57730.17059400282146464No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG51040.1508248381085667No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT47030.13897515940920632No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA46830.1383841529902856No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG42990.12703682974700786No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA41160.12162912101388335No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40220.11885139084495597No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG40100.11849678699360354No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT39900.11790578057468283No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG39830.11769892832806059No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37690.1113751596456089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA47000.038.25476532
GCGGCAC48350.037.1407133
CGGCACC49400.036.29809634
TGGCCGT19400.035.71966670
ACCCGCG53250.033.6633725
CCGTCGC47650.033.6425545
TCGCCGG47800.033.49266448
CCCGTCG48350.033.3454644
GCACCCC54450.033.1760436
CCCCCGA54600.032.91366219
GTTCCGT3500.032.5650331
CACCCCC55800.032.27528837
TAGTCCG3850.032.0162476
CCCCGAC56800.031.89788420
GGCACCC57700.031.52028835
GTTATCG1750.031.3047776
TCAGGAT20900.030.94564470
GATAGGC38200.029.86618840
TTATCGG1950.029.8499077
CCGCGCG61050.029.62269427
Produced by FastQC (version 0.11.5)