FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384058
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA161190.476321623329151No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC156680.46299442858248885No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG151550.44783511393717246No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC134840.39845652763634665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121470.3589477485314968No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT74300.21955888462904596No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47680.14089593027069866No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC39320.11619186195981274No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA34740.10265781496652834No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC34670.1024509627199061No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA34240.10118029891922657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG33800.061.4741864
TCTACGG33950.061.101755
TACGGCC34250.060.463027
CTACGGC34900.059.241476
GCCATAC37100.055.90971411
ACGGCCA37550.055.4220058
CGGCCAT37750.055.1283769
GGGTCTA27500.054.655812
GCGCGGT72700.053.8617139
CCATACC39750.052.78516812
GGCCATA40250.051.70425410
CATACCA40500.051.47036713
GCGTCTA7600.050.4555472
TACCACC41350.050.4123315
CGCGGTG78200.050.1172610
TGGCGCG78550.049.72038715
CTGAACG42700.047.61176722
CCGAACT10800.047.26350467
ATACCAC44750.046.81159214
CGTCTAC9800.046.1071853
Produced by FastQC (version 0.11.5)