FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3426968
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG155640.4541623966141499No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG149270.43557453702514876No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC137170.40026635790004456No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128160.3739748955928389No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC104620.3052844380221817No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG67290.19635432837423636No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA65360.1907225279022156No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT59290.17301007771301044No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT59240.17286417614637778No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG51910.15147500647802956No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT50260.14666025477915173No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA48080.14029894647396765No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG44680.13037763994294665No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA42910.12521272448415044No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41880.1222071522115176No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT41130.12001862871202766No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG40980.11958092401212966No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG40650.1186179736723541No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.1136573204068436No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG35330.10309404698263888No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG34630.10105142504978162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19800.044.55387570
GTTCCGT3950.038.2867051
CGCGGCA47750.036.8623132
GCGGCAC49850.035.4013233
CGGCACC50750.034.72795534
TCGCCGG48750.033.2114948
ACCCGCG54000.032.88500625
CCCGTCG49200.032.77797744
CCGTCGC49300.032.66800745
CCCCCGA54050.032.55093419
GTGCCGT5050.031.9888651
GCACCCC55900.031.81209836
ATGGCCG21650.031.7574269
GGCACCC56750.031.25599135
CCCCGAC57050.030.9803720
CACCCCC57400.030.88151637
CCGCGCG61100.028.98516527
GTCGGTT2150.028.6699094
CGTACTA1950.028.102262
TTCCGTT6100.028.0734672
Produced by FastQC (version 0.11.5)