FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382260
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG151450.4477775215388527No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG144170.4262534518339809No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC134780.39849094983827377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125590.3713197684388545No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC107650.3182783109518488No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG68180.20158119127447327No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA64050.1893704209611325No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT58490.17293170838433475No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT57160.1689994264190216No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG50360.14889452614524015No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT48560.14357264077865098No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA47480.14037950955869744No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG42330.12515300420428943No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41610.12302425005765376No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA41120.12157551459674892No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG40480.1196832886886283No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT40120.11861891161531048No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG39600.11708147806496248No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38160.11282396977169112No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG34810.10291934978387232No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG34450.10185497271055446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19000.044.10275370
CGCGGCA44950.036.54042432
GTTCCGT2550.035.176121
GCGGCAC47800.034.60419533
CGTACTA1700.034.2385062
CGGCACC49250.033.5392834
GTTATCG2450.033.5218476
ATGGCCG20550.032.76201669
TCGCCGG45700.031.96134248
ACCCGCG52150.031.53703125
CCCCCGA52300.031.14145319
TTATCGG2550.030.8652977
GCACCCC53950.030.79247936
CCCGTCG47950.030.78629344
CCGTCGC47800.030.61541645
CGTTCGT1600.030.1871991
CACCCCC55550.029.8682837
CTTACCC10900.029.7129570
GGCACCC55650.029.7008435
CCCCGAC55000.029.6935720
Produced by FastQC (version 0.11.5)