FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135808
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG165750.5285719023613691No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC133790.4266523970855358No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG129780.413864624364757No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC101470.3235848623385105No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT69020.2201027613935547No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG68200.21748780537583934No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62770.20017169418535827No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT59710.19041344368022534No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG57670.18390794334347No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA55980.1785185827703737No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT49730.15858751556217726No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA44290.1412395146641631No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT43240.1378910953731861No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG42290.13486157315754024No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42100.13425566871441108No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG41240.13151315386656326No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG36860.1175454619670592No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG35650.11368680735555237No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG35040.1117415351960324No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT32470.10354588036002203No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC31850.10156871849296896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA47900.039.9934332
GCGGCAC49600.038.56647533
CGGCACC49600.038.51270334
GTTCCGT2750.037.717611
CCGTCGC49550.036.3791845
TCGCCGG49800.036.26692648
GCACCCC55400.035.13528436
CCCGTCG51900.035.0491544
CCCCCGT52400.034.6777242
CCCCCGA54050.034.63387319
CGTACGT1800.034.4744219
ACCCGCG56600.034.28889525
GGCACCC57200.033.5904235
CCCCGAC56100.033.2549520
CACCCCC58550.033.20978537
CAGTCCG57400.032.585476
AGTCCGC59850.032.517747
CCCGACC57800.032.40759321
CCGCGCG60150.032.06122227
AGGACCG4800.031.78289670
Produced by FastQC (version 0.11.5)