FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135808
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA174430.5562521685001123No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC77880.24835704226789396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62630.1997252382798947No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC59410.18945675245423188No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA37110.11834270465538707No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT34580.11027460864950916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT35400.050.0029349
TGGCGCG37850.046.6700815
CGCGGTG38450.046.30520610
GGGGACT41000.045.1478041
GGACTCT43650.044.176453
TCTGGTC42350.044.15521610
ACTCTTC43000.044.1277545
GGGACTC44950.041.6731452
TTCTGGT45900.041.1153039
GACTCTT48150.041.052944
CTGGTCC45600.040.84800711
CTACTCG44400.040.58053237
TACTCGG44700.040.2434838
CGGGACT9600.039.2845651
GTAGTCC46100.039.27460527
CGCGTGC46250.038.80818
GGCGCGT46450.038.5482516
GCCGGTT5900.038.5193523
TAGTCCC47500.038.28093328
CGGTTCA5850.038.263635