FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115218
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG163730.5255811952807156No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC132000.4237263652174583No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG127980.41082197136765386No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC96980.3113104765059781No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT69000.2214933272727623No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG67460.21654985301189195No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60680.19478572607117703No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG58030.18627909828461442No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT57850.18570128960477245No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA57450.1844172703162347No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT49930.16027770769172495No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA43910.14095321739923178No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT43210.13870618364429071No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG41890.13446891999211613No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40980.13154777611069274No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG40290.1293328428379651No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG38570.12381155989725277No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG35540.1140851137865793No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG35510.11398881233993897No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT32120.10310674886958153No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.1021437344031782No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC31260.10034610739922535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA45750.038.519132
GCGGCAC47950.037.0567933
CGGCACC47750.037.00935434
CCGTCGC46750.035.7986645
TCGCCGG47900.034.85774248
GCACCCC51750.034.4561436
CCCGTCG49550.034.1025844
CCCCCGT49500.033.67307742
GGCACCC54600.032.63434635
CACCCCC54950.032.4727437
GTACGGG19200.032.4629635
CCCCCGA53800.032.09287319
TACGGGT20000.031.6809846
ACCCGCG56250.031.54330425
TAGTCCG2850.031.4151656
ACGATCG553.2940472E-431.305326
AGTCCGC57000.031.2943347
CCCCGAC55150.031.24881620
CAGTCCG54700.030.9733816
TTATCGG2250.030.6096467
Produced by FastQC (version 0.11.5)