FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115218
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA173950.5583878881028551No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC75740.24312905228462342No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60450.19404741498026784No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC59480.1909336682055638No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA37530.12047310974705464No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT33950.10898113711464175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT34900.050.015699
CGCGGTG37100.047.328210
TGGCGCG37300.046.98137315
GGGGACT45600.046.575591
TCTGGTC45500.046.00615310
GGACTCT47250.045.691813
ACTCTTC46250.045.41415
GGGACTC49800.043.992842
TTCTGGT48400.043.676399
CCGAACT5400.043.5307867
GACTCTT51300.042.084564
CTGGTCC49500.042.0102711
CGGGACT11050.041.3879931
CTACTCG44000.040.4683237
TACTCGG44100.040.31287438
TCTTCTG53750.039.843427
TGGTCCC52350.039.78830312
CGGCAAC5800.039.425631
GGCGCGT44800.039.42358416
CGCGTGC45200.039.01609818