FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102871
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA157670.508142297891211No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC74620.24048695546801654No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59210.19082327302681934No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC55140.17770638869614624No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA35210.1134755521579853No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT32570.10496730286241356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT36350.051.702489
CGCGGTG37400.050.2509410
TGGCGCG38750.048.4963415
ACTCTTC41550.046.392435
GCCGGTT6100.045.1371543
GGACTCT44250.044.489423
TCTGGTC42950.044.39864710
GGGGACT42300.043.962681
CGGGACT11150.043.562511
CTACTCG43500.043.33291237
TACTCGG44250.042.6991638
CCGAACT6450.042.52585267
GGGACTC45550.042.1693572
GACTCTT47800.041.8333134
GGCGCGT45450.041.7232116
TTCTGGT46000.041.3051649
CTGGTCC46150.041.02174411
GTAGTCC46150.040.867327
CGTGCCT46550.040.5659120
ACTCGGG46800.040.39037739