FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3101732
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG137300.4426559096659544No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51460.16590730598259296No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG41030.13228093207279032No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34950.11267898064694178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41450.036.3672932
TCGCCGG40350.035.42825748
CCCGTCG41100.034.66084744
GCGGCAC43800.034.50264433
ACCCGCG44700.033.42990525
CCGTCGC42550.033.4054345
CGGCACC46300.032.4943234
CCGCGCG48950.030.89754927
CCCGCGC49450.030.50511426
GCACCCC50050.030.41566536
CCCCCGA50050.030.37774719
CCCCGAC50050.030.31295620
AGGAGGG70850.029.1158270
CACCCCC52700.028.8300937
CGTCGCC51200.028.04211446
CCCCCGT52100.027.46447442
ACCGACG5900.027.0661530
GTCGCCG53150.026.9529847
GGCACCC57500.026.28968235
CCCGACC58250.026.16846321
Produced by FastQC (version 0.11.5)