FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205585
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG147670.46066474606039143No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51720.16134340533787125No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG43740.1364493532381765No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37620.11735767418427526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG43400.035.34461648
CGCGGCA45800.035.27287332
GCGGCAC47650.034.27333533
CCCGTCG45200.033.90402644
ACCCGCG49000.033.27273625
CCGTCGC46300.033.03005645
CGGCACC50450.032.0991734
CCCCCGA53350.030.5888919
AGGAGGG73850.029.98614770
CCGCGCG54850.029.92220327
GCACCCC54350.029.8051736
CCCCGAC54600.029.7686620
CCCGCGC55450.029.46912626
TAGTCCG2650.028.747246
CACCCCC57400.028.59378437
CCCCCGT54550.028.40132142
CGTCGCC54850.028.01364746
GTCGCCG55450.027.78078547
GCGCGGG55900.027.35207462
CCCGACC61600.026.4470821