FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205585
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54210.16911109828627224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51070.1593156943272445No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC37940.11835593191258383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT28050.045.7717179
TGGCGCG29200.043.96769315
CGGTATA2200.041.0536731
TACTCGG34500.038.19589238
CTACTCG34700.037.96739637
AGGATCG34800.036.7610759
CGCGGTG35450.036.60759710
ACTCGGG37000.036.28484739
CGCGTGC36650.036.26843318
CGTGCCT36500.035.96352820
GATCGCT35450.035.91250261
GCGTGCC36850.035.7070119
GTGGCGC36450.035.6021614
GGGGTAG15750.034.8479961
GGCGATA2900.034.737721
TAGTCCC38300.034.32862528
GTAGTCC38050.034.2704627
GGGGGGA65200.033.139361
GGCGCGT39900.033.13088616
CGGTATT2950.032.9713941
Produced by FastQC (version 0.11.5)