FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3807898
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG157230.41290496751751227No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96340.25300047427740974No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC89270.23443380048520207No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74690.19614495976520377No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG73400.19275726398133564No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC70790.18590308879071865No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49600.13025558982934943No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42520.11166265482951487No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT40640.10672554779566049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA50050.036.22638332
GCGGCAC52100.034.9453733
TCGCCGG50100.034.5327748
CCGTCGC50700.033.91496345
CGGCACC54350.033.26202434
CCCGTCG52050.033.07803744
ACGATCG553.324462E-431.256516
ACCCGCG58800.030.91093425
GCACCCC59350.030.83938836
CCCCCGA59900.030.39662619
CGTACGT2500.028.91184419
CACCCCC64150.028.65855637
AGTCCGC65400.028.1786527
GGCGATC10900.028.05231570
CCCCGAC65250.027.96703320
CAGTCCG64000.027.8280626
GGCACCC65650.027.70718435
ATGCCCG9950.027.65760870
GTACGGG11800.027.0974465
CGATCTA2850.026.96230747