FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832116
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG172510.45016904498715593No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98220.256307481297539No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC87160.22744614202701588No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG79330.20701356639517177No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG78310.2043518515619047No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC72170.1883293720753756No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT50590.13201583668135305No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45000.11742859558531107No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT41800.10907811767702232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51200.035.415732
AGGACCG6550.034.24162770
GCGGCAC53250.034.06932433
TCGCCGG52150.033.26518648
CCCGTCG53550.032.87787644
CCGTCGC53500.032.6730845
CGGCACC56300.032.4324934
GCACCCC60100.030.53700836
CCCCCGA59900.030.45166219
ACCCGCG62650.029.176925
GTTATCG1800.028.6497156
CACCCCC65300.028.22718437
GTACGGG12600.027.8311525
GGCACCC66650.027.67206635
GATAGGC28600.027.47590640
AGTCCGC67900.027.4429667
CCCCGAC67300.027.26603720
TACGGGT12750.027.2340816
CAGTCCG66750.026.9371686
GCATTAG29950.026.28695112