FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3926517
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG173420.4416636932935729No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102000.2597722103329745No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC89950.22908343450442215No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG81630.207894171857654No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG76830.19566959725374933No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC72900.18566072679680234No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT52190.1329167809537053No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45100.11486006554918773No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT42940.10935900697743063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52750.036.9435732
GCGGCAC55450.035.22251533
CGGCACC55900.035.01352734
TCGCCGG53650.034.33216548
CCCGTCG54750.033.3521744
CCGTCGC55700.032.55624445
GCACCCC61100.032.41010736
TAGTCCG4450.031.6810866
CCCCCGA62300.031.55096419
GTTATCG2300.031.3955216
ACCCGCG63650.030.84379425
AGGACCG6300.030.07296470
CACCCCC66050.029.99625637
GGCACCC66850.029.39747235
CCCCGAC70000.028.13888520
AGTCCGC72300.027.632117
TACGGGT12750.027.2387666
CGTCGCC68200.026.86468746
CAGTCCG70150.026.4692926
CGTACGT2350.026.36751619