FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3889293
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA102170.26269555932144995No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97870.2516395653400245No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC44870.1153680116154787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT24350.043.8817569
TGGCGCG25300.041.96241815
GGGGACT41300.037.4998931
CTACTCG28100.037.1608537
TCTGGTC31550.036.80780410
TACTCGG28700.036.5338638
CCGAACT5350.036.1636867
CGGGTAG5350.036.105051
ACTCTTC34000.035.2684145
AGTACGC5700.034.89390664
GGACTCT37050.034.774883
CGGTCTA1800.034.4932171
CGGTAAG3250.033.9625551
GGGACTC39300.033.9204642
GTACGCA5350.033.90071565
CGCGGTG31800.033.81736810
CGGGACT9300.033.7514271
GGGGTAG15850.033.7315371
AGGATCG30000.033.66839659
GACTCTT38350.033.596074
Produced by FastQC (version 0.11.5)