FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080951
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC96320.23602341709077126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88760.21749832330748395No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC66550.16307473429600108No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA48080.11781567580693815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT47700.048.2299359
TGGCGCG50200.046.2413815
CGCGGTG52250.044.69078410
CGGCATA3450.043.0866361
CCGAACT7700.041.10535467
CTACTCG57800.039.1788437
TACTCGG58850.038.26363838
GGCGCGT61050.037.9094516
GTAGTCC61300.037.77000427
CGTGCCT61050.037.6764220
TAGTCCC61700.037.59597428
GCGTGCC62800.036.93692419
TGTAGTC63450.036.42987426
CGGCAAT4700.036.0405541
ACTCGGG63100.035.92453839
CGCGTGC64700.035.84473418
AGGATCG63800.035.39828559
CGGGTAG5900.035.1554341
CGGTATA1700.034.569511
CCTGTAG68150.034.29897724
Produced by FastQC (version 0.11.5)