FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4103785
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103300.25171884004644496No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88380.21536215956732627No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC71840.17505790386192258No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA49710.12113207685100463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT46900.050.7314649
TGGCGCG50450.047.09225515
CGCGGTG52500.045.2523110
CGGCATA2650.043.010921
CCGAACT8100.040.40604467
CTACTCG60850.039.7728937
TACTCGG60900.039.7612938
GTAGTCC63100.038.34740427
CGTGCCT62250.038.16894520
TAGTCCC63400.038.01869628
ACTCGGG64550.037.64055639
TGTAGTC64550.037.6328126
GGCGCGT64700.037.0928416
GCGTGCC64700.036.7634119
GGGGTAG19600.036.6537131
CGCGTGC65350.036.53991718
AGGATCG65000.035.5121359
GATCGCT65500.035.50555461
CCTGTAG67800.035.39642724
CGAACTT9800.034.8168968
Produced by FastQC (version 0.11.5)